A leading global manufacturer of complete solutions for epigenetics research, biological sample preparation, and diagnostics assays
STATE-OF-THE ART SHEARING DEVICES
FOR SAMPLE PREPARATION
Plus many other biological, chemical and pharmaceutical applications in which highly effective and precise sample preparation is required including in the use of techniques such as:
The Diagenode Bioruptor® sonication systems are all-in-one shearing devices with advanced temperature control and cited in more than 2000 peer reviewed publications!
Key Product Features:
The Bioruptor* uses state-of-the-art ultrasound technology to give the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Also provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.
FFPE nucleic acid extraction
The Bioruptor* is a superior method for removing the paraffin and rehydrating FFPE tissues in just one solvent-free step followed by a mild crosslink reversal to preserve DNA and RNA integrity.
The Bioruptor* (and the Megaruptor) provides superior sample yields, appropriate fragment size and consistency for sensitive and reliable for Next-Generation Sequencing (NGS) results.
The Bioruptor* provides unbiased RNA shearing for best cDNA synthesis and ensures high quality Next-Generation Sequencing.
The Bioruptor* provides high throughput and reproducibility and eliminates the need for direct contact to prevent sample contamination. The precise temperature control preserves the lipids from damage through overheating or oxidation.
The Bioruptor* delivers high quality protein by ensuring efficient disruption and homogenization of tissues and cultured cells obtained in just one step essential for obtaining high quality and yields of proteins for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification.
*The Bioruptor® uses ACT (Adaptive Cavitation Technique) to create focused mechanical stress to shear DNA or Chromatin or to disrupt cells or tissues. The ultrasound waves pass through the sample, expanding and contracting the liquid. During expansion, negative pressures pull the molecules away from one another and form a cavity or bubble. The bubble continues to absorb energy until it can no longer sustain itself and then implodes, producing intense focused shearing forces, which disperse or break biomolecules.